Phaeoacremonium parasiticum (Ajello et al.) Gams et al., anamorph
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1. To thaw a frozen ampoule, place in a 2530 °C water bath, until just thawed (approximately 5 minutes). Immerse the ampoule just sufficient to cover the frozen material. Do not agitate the ampoule.
2. Immediately after thawing, wipe down ampoule with 70% ethanol and aseptically transfer at least 2-3 agar cubes in the content onto a plate or broth with medium recommended.
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4. Inspect for growth of the inoculum/strain regularly. The sign of viability is noticeable typically after 1-2 days of incubation. However, the time necessary for significant growth will vary from strain to strain.
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McNeil CJ, et al. Brain abscess caused by Phaeoacremonium parasiticum in an immunocompromised patient. J. Clin. Microbiol. 49: 1171-1174, 2011.