Smittium gravimetallum Ferrington, Lichtw. et Hayford
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1. To thaw a frozen ampoule, place in a 2530 °C water bath, until just thawed (approximately 5 minutes). Immerse the ampoule just sufficient to cover the frozen material. Do not agitate the ampoule.
2. Immediately after thawing, wipe down ampoule with 70% ethanol and aseptically transfer 10 microliter (or any amount desired up to all) of the content onto a plate or broth with medium recommended.
3. Incubate the inoculum/strain at the temperature and conditions recommended.
4. Inspect for growth of the inoculum/strain regularly. The sign of viability is noticeable typically after 1-2 days of incubation. However, the time necessary for significant growth will vary from strain to strain.
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Ferrington LC, Lichtwardt RW, Hayford B. Smittium gravimetallum (Trichomycete: Harpellales), a new species of gut fungus from Dicrotendipes fumidus (Johannsen) (Diptera: Chironomidae) in a metal-polluted stream. In: Late 20th Century Research on Chironomidae: an Anthology from the 13th International Symposium on Chironomidae, pp. 253-257. Ed. O. Hoffrichter. Shaker Verlag, Aachen, 2000.