Crepidotus nephrodes (Berk. & M.A. Curtis) Sacc.
1. To thaw a frozen ampoule, place in a 2530 °C water bath, until just thawed (approximately 5 minutes). Immerse the ampoule just sufficient to cover the frozen material. Do not agitate the ampoule.
2. Immediately after thawing, wipe down ampoule with 70% ethanol and aseptically transfer 10 microliter (or any amount desired up to all) of the content onto a plate or broth with medium recommended.
3. Incubate the inoculum/strain at the temperature and conditions recommended.
4. Inspect for growth of the inoculum/strain regularly. The sign of viability is noticeable typically after 1-2 days of incubation. However, the time necessary for significant growth will vary from strain to strain.
Additional, updated information on this product may be available on the ATCC web site at www.atcc.org.
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Once you have the necessary permit, email the permit to [email protected] with a reference to both your account and sales order numbers. Once received, your permit will be reviewed, and this item will be released for shipment if all requirements are met. If you need assistance with your order, please contact our Customer Care team or your applicable distributor.
Aime MC. Intercompatibility tests and phylogenetic analysis in the Crepidotus Sphaerula group complex: Concordance between ICGs and nuclear rDNA sequences highlight phenotypic plasticity within Appalachian species. In: Fungi in Forest Ecosystems: Systematics, Diversity, and Ecology. CL Cripps, Ed. The New York Botanical Garden. pp. 71-80, 2004.