Crepidotus appalachianensis Hesler et A.H. Sm.
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1. To thaw a frozen ampoule, place in a 2530 °C water bath, until just thawed (approximately 5 minutes). Immerse the ampoule just sufficient to cover the frozen material. Do not agitate the ampoule.
2. Immediately after thawing, wipe down ampoule with 70% ethanol and aseptically transfer 10 microliter (or any amount desired up to all) of the content onto a plate or broth with medium recommended.
3. Incubate the inoculum/strain at the temperature and conditions recommended.
4. Inspect for growth of the inoculum/strain regularly. The sign of viability is noticeable typically after 1-2 days of incubation. However, the time necessary for significant growth will vary from strain to strain.
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Aime MC, Miller OK. Delayed germination of basidiospores in temperate species of Crepidotus (Fr.) Staude. Can. J. Bot. 80: 280-287, 2002.
Aime MC. Intercompatibility tests and phylogenetic analysis in the Crepidotus Sphaerula group complex: Concordance between ICGs and nuclear rDNA sequences highlight phenotypic plasticity within Appalachian species. In: Fungi in Forest Ecosystems: Systematics, Diversity, and Ecology. CL Cripps, Ed. The New York Botanical Garden. pp. 71-80, 2004.