Agaricus cupressophilus Kerrigan
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ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
1. To thaw a frozen ampoule, place in a 25-30°C water bath, until just thawed (approximately 5 minutes). Immerse the ampoule just enough to cover the frozen material. Do not agitate the ampoule.
2. Immediately after thawing, aseptically transfer the contents of the vial onto appropriate solid or liquid medium.
3. Incubate cultures at recommended temperature.
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Kerrigan RW, Callac P, Parra LA. New and rare taxa in Agaricus section Bivelares (Duploannulati). Mycologia 100: 876-892, 2008.