Dictyostelium discoideum Raper
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ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.
ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
1. Prepare media by streaking center of agar surface with a large X of Escherichia coli (ATCC 23437) and incubating at 25°-30°C one day before inoculating organism. Several replicates are suggested for optimum results.
2. To thaw frozen ampoule, place in a 37°C water bath, immerse ampoule to depth of one millimeter above the level of frozen material in the ampoule. Keep ampoule immersed until material is thawed but no more than 3 minutes. Do not agitate or vortex the ampoule.
3. Immediately after thawing, wipe down ampoule with 70% ethanol and aseptically transfer one loop full of contents onto center of X grown out with Escherichia coli.
4. Incubate the plates at the temperature recommended.
5. Allow culture to incubate for 2-5 days. Visually inspect by inverting plate under 10X objective. Look for swarms of amoebae feeding on bacteria and the initials of fruiting bodies and/or fruiting bodies rising from the surface of the agar.
The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid. Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.
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