Phytophthora trifolii E.M. Hansen & D.P. Maxwell
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1. To thaw a frozen ampoule (or plastic vial), place it in a water bath maintained at 25-30°C until it just thawed (around 5 minutes)." Immerse the ampule just enough to cover the frozen material. Do not agitate the ampule.
2. Immediately after thawing, aseptically transfer the contents of the vial onto appropriate moist solid or liquid medium.
3. Incubate cultures at recommended temperature.
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Gallegly ME, Hong C. Phytophthora; identifying species by morphology and DNA fingerprints. APS Press, St. Paul, Minnesota, USA, 2008.