Phytophthora pseudotsugae Hamm et Hansen
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1. To thaw a frozen ampule, place in a 30°C water bath, until just thawed (approximately 5 minutes). Immerse the ampule just enough to cover the frozen material. Do not agitate the ampule.
2. Immediately after thawing, aseptically transfer the contents of the vial onto appropriate solid or liquid medium.
3. Incubate cultures at recommended temperature.
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Gallegly ME, Hong C. Phytophthora; identifying species by morphology and DNA fingerprints. APS Press, St. Paul, Minnesota, USA, 2008.