Gaeumannomyces graminis var. graminis (Saccardo) von Arx et Olivier, teleomorph
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1. To thaw a frozen ampoule, place in a 2530 °C water bath, until just thawed (approximately 5 minutes). Immerse the ampoule just sufficient to cover the frozen material. Do not agitate the ampoule.
2. Immediately after thawing, wipe down ampoule with 70% ethanol and aseptically transfer at least 50 µl (or 2-3 agar cubes) of the content onto a plate or broth with medium recommended.
3. Incubate the inoculum/strain at the temperature and conditions recommended.
4. Inspect for growth of the inoculum/strain regularly. The sign of viability is noticeable typically after 5-6 days of incubation. However, the time necessary for significant growth will vary from strain to strain.
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Saleh AA, Leslie JF. Cephalosporium maydis is a distinct species in the Gaeumannomyces-Harpophora species complex. Mycologia 96: 1294-1305, 2004.