Phytophthora ramorum Werres et al.
1. To thaw a frozen ampoule, place in a 2530 °C water bath, until just thawed (approximately 5 minutes). Immerse the ampoule just sufficient to cover the frozen material. Do not agitate the ampoule.
2. Immediately after thawing, wipe down ampoule with 70% ethanol and aseptically transfer at least 50 µl (or 2-3 agar cubes) of the content onto a plate or broth with medium recommended.
3. Incubate the inoculum/strain at the temperature and conditions recommended.
4. Inspect for growth of the inoculum/strain regularly. The sign of viability is noticeable typically after 5-6 days of incubation. However, the time necessary for significant growth will vary from strain to strain.
Additional, updated information on this product may be available on the ATCC web site at www.atcc.org.
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Parke JL, et al. Detection of Phytophthora ramorum blight in Oregon nurseries and completion of Koch's postulates on Pieris, Rhododendron, Viburnum, and Camellia. Plant Dis. 88: 87, 2004.