Phytophthora ramorum Werres et al.
Frozen ampules packed in dry ice should either be thawed immediately or stored in liquid nitrogen vapor. If liquid nitrogen storage facilities are not available, frozen ampules may be stored at or below -70°C for approximately one week. Do not under any circumstance store frozen ampules at refrigerator freezer temperatures (generally -20°C). Storage of frozen material at this temperature may result in the death of the culture.
1. To thaw a frozen ampule, place in a 30°C water bath, until just thawed (approximately 4 minutes). Immerse the ampule just enough to cover the frozen material. Do not agitate the ampule.
2. Immediately after thawing, aseptically transfer the contents of the vial onto appropriate solid or liquid medium.
3. Incubate cultures at recommended temperature.
Additional, updated information on this product may be available on the ATCC web site at www.atcc.org.
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