Tyromyces formosanus Chang et Chou
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1. To thaw a frozen ampoule, place in a 2530 °C water bath, until just thawed (approximately 5 minutes). Immerse the ampoule just sufficient to cover the frozen material. Do not agitate the ampoule.
2. Immediately after thawing, wipe down ampoule with 70% ethanol and aseptically transfer 10 microliter (or any amount desired up to all) of the content onto a plate or broth with medium recommended.
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Chang TT, Chou WN. Ceriporiopsis microporus and Tyromyces formosanus, two new polypore species from Taiwan. Mycol. Res. 103: 674-676, 1999.