Agaricus bisporus (Lange) Imbach, teleomorph
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1. To thaw a frozen ampule, place it at room temperature or in 30°C water bath, until just thawed (around 1.5 min).
2. Immerse the ampule just sufficient to cover the frozen material.
3. Immediately after thawing, aseptically transfer the culture into a test tube or plate with medium recommended.
4. Incubate the test tube or plate at the temperature recommended.
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Galan B, et al. Production, purification, and properties of an endo-1,3-beta-glucanase from the basidiomycete Agaricus bisporus. Curr. Microbiol. 38: 190-193, 1999.