Alternaria alternata (Fries) Keissler
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1. To thaw a frozen ampule, place in a 30°C water bath, until just thawed (approximately 4 minutes). Immerse the ampule just enough to cover the frozen material. Do not agitate the ampule.
2. Immediately after thawing, aseptically transfer the contents of the vial onto appropriate solid or liquid medium.
3. Incubate cultures at recommended temperature.
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Aradhya MK, et al. Genetic variability in the pistachio late blight fungus, Alternaria alternata. Mycol. Res. 105: 300-306, 2001.