Aspergillus nidulans (Eidam) Winter
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ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
1. Using sterile technique, transfer one or more agar blocks bearing the fungus to tubes or plates of recommended medium. New culture may grow more quickly if mycelium is placed in contact with the fresh medium.
2. Incubate cultures at recommended temperature.
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Jesus Aguirre, personal communication