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ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
Thawing the frozen ampoule at the recommended temperature and time is very important for better recovery. Keep culture moist during incubation. It is recommended that this strain be initially started in a nutrient broth containing trace amount of biotin and thiamine (ATCC® Medium 2232) and transferred to ATCC® Medium 1970 once growth is observed. ATCC® Medium 1970 should be used for any subsequent subculture attempts.Additional information on this culture is available on the ATCC® web site at www.atcc.org.
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Samen FA, et al. Virulence variation in a single zoospore population of Phytophthora infestans. Phytopathology 90: S1, 2000.