Pseudallescheria angusta (Malloch et Cain) McGinnis et al.

MYA-3634 ^™

An ampoule containing viable cells (yeast cells, spores, or agar cubes with mycelia) suspended in cryoprotectant.

Product category: Fungi
Strain designation: SC1
Type strain: No
Isolation source: Heart
Product format: Frozen
Storage conditions: -80°C or colder

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Documentation

Product sheet

Characterization data

BSL 1

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

General

Specific applications: Antifungal susceptibility testing RefGhannoum MA, et al. Interlaboratory study of quality control isolates for a broth microdilution method (modified CLSI M38-A) for testing susceptibilities of dermatophytes to antifungals. J. Clin. Microbiol. 44: 4353-4356, 2006. RefEspinel-Ingroff A, et al. Quality control and reference guidelines for CLSI broth microdilution susceptibility method (M38-A document) for amphotericin B, itraconazole, posaconazole, and voriconazole. J. Clin. Microbiol. 43: 5243-5246, 2005.
Preceptrol: No

Characteristics

Morphology: After 5 days cleistothecia form, immersed, membranaceous, spherical, light brown to black. Erect synnemata may be present, producing broadly clavate, subhyaline or pale brown conidia. Conidiogenous cells also arising from undifferentiated hyphae, cylindrical, producing slimy heads of 1-celled, smooth-walled, subhyaline to brown, subspherical to elongate conidia.
Comments: This organism is a CLSI control strain for antimicrobial susceptibility testing.
This fungus was re-identified as Pseudallescheria angusta based on the multigene DNA sequence comparison.

Handling information

Medium

ATCC Medium 325: Malt extract agar (Blakeslee's formula)

ATCC Medium 336: Potato dextrose agar (PDA)

ATCC Medium 200: YM agar or YM broth

Temperature

25-30°C

Atmosphere

Aerobic

Handling procedure

Frozen ampoules packed in dry ice should either be thawed immediately or stored in liquid nitrogen. If liquid nitrogen storage facilities are not available, frozen ampoules may be stored at or below -70°C for approximately one week. Do not under any circumstance store frozen ampoules at refrigerator freezer temperatures (generally -20°C). Storage of frozen material at this temperature will result in the death of the culture.

To thaw a frozen ampoule, place in a 25°C to 30°C water bath, until just thawed (approximately 5 minutes). Immerse the ampoule just sufficient to cover the frozen material. Do not agitate the ampoule.
Immediately after thawing, wipe down ampoule with 70% ethanol and aseptically transfer at least 50 µL (or 2-3 agar cubes) of the content onto a plate or broth with medium recommended.
Incubate the inoculum/strain at the temperature and conditions recommended. Inspect for growth of the inoculum/strain regularly. The sign of viability is noticeable typically after 5-7 days of incubation. However, the time necessary for significant growth will vary from strain to strain.

Handling notes

This organism is a CLSI control strain for antimicrobial susceptibility testing.
Additional, updated information on this product may be available on the ATCC^® web site at www.atcc.org.

Quality control specifications

Sequenced data

18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence

GGTCTCCGTTGGTGAACCAGCGGAGGGATCATTACAGAGTTACTACTCCAAACCCATTGTGAACCTTACCTATGTTCTGTTGCCTCGGCGGCGTGGTCAGCGCCCCTCTGAAAAGAGGACGATGCCCTCCCGCCGGCAGCACCAAACTCTTGAATTTTACAGCGGATTACAATTCTGATTTGAAAACAAAAAACAAGTTAAAACTTTCAACAACGGATCTCTTGGTTCTGGCATCGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCGGCAGTAATCTGCCGGGCATGCCTGTCCGAGCGTCATTTCAACCCTCGAACCTCCGTTTCCTTAGGGAAGCTCAGGGTCGGCGTTGGGGCGCTACGGCGAGTCTTCGCGACCCCCGTAGGCCCTGAAATACAGTGGCGGTCCCGCCGCGGTTGCCTTCTGCGTAGTAAGTCTCTTTTGCAAGCTCGCATTGGGTCCCGGCGGAGGCCTGCCGTCAAACCTATAACTTCAGATGGTTTGACCTCGGATCAGGTAGGGTTACCCGCTGAACTTAAGCATATCAATAA

D1D2 region of the 28S ribosomal RNA gene

ATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCTCAGTAACGGCGAGTGAAGCGGCAACAGCTCAAATTTGAAATCTGGCGGCCTCTGGGCCGTCCGAGTTGTAATTTGAAGAGGATGCTTTTGGCGAGGTGCCTTCCGAGTGCCCTGGAACGGGACGCCACAGAGGGTGAGAGCCCCGTATGGTTGGACGCCGAGCCTCTGTAAAGCTCCTTCGACGAGTCGAGTAGTTTGGGAATGCTGCTCAAAATGGGAGGTAAACCCCTTCTAAAGCTAAATACTGGCCAGAGACCGATAGCGCACAAGTAGAGTGATCGAAAGATGAAAAGCACTTTGAAAAGAGAGTTAAACAGCACGTGAAATTGTTGAAAGGGAAGCGCTTGCGACCAGACTTGTGCTCGTCGAATCAGCCGCCGCTCGTCGGCGGCGCACTTCGGCGGGCTCAGGCCAGCATCAGTTCGCTGCAGGGGGAGAAAGGCGATGGGAATGTGGCTCTTCGGAGTGTTATAGCCCGCCGCGCAATACCCCTCGGCGGACTGAGGACCGCGCATCTGCAAGGATGCTGGCGTAATGGTCGTCAGCGAC

Beta-tubulin gene (bTub)

CCCCCCTCCCATTCCGAGCTCGCGATATATCCTATCTCAGGATCCATCTCGACCTTCATCATCGTCACTCGATGCTCTTTTGCCATCGTCTGGCGGTGAAGCTGTCGTCGGGCAATATTGCGACGCGTTCAAGCTTGCCTCCTTCAACGGCGGTGCACTTGGTGACCCCGCCCACAGGACGAGACATAGCTTGAAGAGATGCAGCCTCGTCCAATAAACCATCCAATGGGTTAAGTTTCGAATGCTAACGTATATTTTCTTTTTCCTCGTGTACAGGTTCATCTGCAGACAGGCCAGTGCGTAAGTAAATTCTTGAGGACCCTATGTCCTTTTCCCCCCTGCTTCCATTTCCCTTCTGGGCCATGATCTTGTCTGCGGGGCTAACGTTTATTTTTGCCCCCCTTTCTAGGGAAACCAAATTGGTGCTGCCTTCTGGTGCGTACATTTTTCCCGACGACGCGCGATCGAGAAACGGATGCGAATATAATTCTTCTGTGCGAGATATTTCTATTTGATGGACAGAGTACTTATCGTATTTTTAGGCAAACCATTTCTGGCGAGCACGGTCTTGATAGCAACGGAGTGTACGGAACCCCCTCATCCCTTTCCCCCCCAAATGCCAGTCACATCGACTGGTGCTGCTCTCACATTCACGGCAGACACTGATTGTCCCTTATACGTCAAAGTTACAATGGCACTTCTGAACTCCAGCTTGAGCGCATGAGCGTCTACTTCAACGAGGTAAGTTTTTGGTTCAAACCATGGGCTGGTTGGTGTGGTGTCATCCGGCCTCCGTTGTTGCAGAAAATACTAACGTATTGGTTACCTCCACATTATAGGCTTCGGGCAACAAATATGTTCCTCGCGCTGTCCTTGTAGATCTCGAGCCCGGTACCATGGACGCCGTCCGT

History

Deposited as: Scedosporium apiospermum
Synonyms: Petriellidium angustum Malloch et Cain
Depositors: TJ Walsh
Chain of custody: ATCC <-- T J Walsh
Type of isolate: Human
Special collection: NSF - Mycology

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Intended use

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

Warranty

The product is provided 'AS IS' and the viability of ATCC^® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid. Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

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While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

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References

Curated Citations

Reference Method for Broth Dilution Antifungal Susceptibility Testing of Filamentous Fungi: Approved Standard - 2nd Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M38-A2.

Ghannoum MA, et al. Interlaboratory study of quality control isolates for a broth microdilution method (modified CLSI M38-A) for testing susceptibilities of dermatophytes to antifungals. J. Clin. Microbiol. 44: 4353-4356, 2006.

Espinel-Ingroff A, et al. Quality control and reference guidelines for CLSI broth microdilution susceptibility method (M38-A document) for amphotericin B, itraconazole, posaconazole, and voriconazole. J. Clin. Microbiol. 43: 5243-5246, 2005.

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