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Escherichia coli (Migula) Castellani and Chalmers (ATCC® BAA-2216™)

Strain Designations: 99-3071  /  Type Strain: no  /  Biosafety Level: 2

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Classification Enterobacteriaceae, Escherichia
Strain Designations 99-3071
Isolation
Stool
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Antigenic Properties Serotype O145:H34
Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol® no
Type Strain no
Antigenic Properties Serotype O145:H34
Comments This strain was confirmed by PCR to carry the eae gene but not the stx1 and stx2 genes (stx1-, stx2-, eae+).
Non-toxigenic

Depositor-provided information: negative control for STEC virulence genes; genotype ipaH-, stx1-, stx2-, eae+, E-hly-

Growth on Novobiocin (10 mg/L)
Growth on Tellurite (0.8 mg/L)
Medium ATCC® Medium 3: Nutrient agar or nutrient broth
Growth Conditions
Temperature: 37°C
Atmosphere: Aerobic
Name of Depositor N Strockbine
Isolation
Stool
References

Nancy A Strockbine, personal communication

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
SDS
SDS
Other Documentation
Other Document
Microbial Characterization Data