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Escherichia coli (Migula) Castellani and Chalmers (ATCC® BAA-2216™)

Strain Designations: 99-3071  /  Type Strain: no  /  Biosafety Level: 2

Classification Enterobacteriaceae, Escherichia
Strain Designations 99-3071
Isolation
Stool
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Antigenic Properties Serotype O145:H34
Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol® no
Type Strain no
Antigenic Properties Serotype O145:H34
Comments This strain was confirmed by PCR to carry the eae gene but not the stx1 and stx2 genes (stx1-, stx2-, eae+).
Non-toxigenic

Depositor-provided information: negative control for STEC virulence genes; genotype ipaH-, stx1-, stx2-, eae+, E-hly-

Growth on Novobiocin (10 mg/L)
Growth on Tellurite (0.8 mg/L)
Medium ATCC® Medium 3: Nutrient agar or nutrient broth
Growth Conditions
Temperature: 37°C
Atmosphere: Aerobic
Name of Depositor N Strockbine
Isolation
Stool
References

Nancy A Strockbine, personal communication