Distribution host: Escherichia coli
HB101 (ATCC 33694)
Size (kb): 4.7109999656677250
DESCRIPTION OF VECTOR:
Intact vector size: 4.711
Type of vector: plasmid
Cloning sites: HindIII SalI BamHI ClaI SmaI KpnI SacI
Polylinker sites: HindIII SalI BamHI [tk-promoter luc+ SV40 poly (A)] ClaI
SmaI KpnI SacI
Other unique sites: NarI BclI FseI ClaI SmaI KpnI SacI PvuII BsaI BglI FspI PvuI
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
MCS: HindIII...BamHI, ->, 1-36
promoter: TK, 37-200
reporter group: luciferase (luc+), ->, 238-1890
terminator: SV40 late polyadenylation, 1922-2143
MCS: ClaI...SacI, 2146-2168
replicon: pMB1, 2587-2587
marker(s): ampR, <-, 3345-4205
terminator: SV40 large T-antigen polyadenylation, 4337-4708
Vector: ptkLUC+ (plasmid)
Promoters: Promoter TK
Construct size (kb): 4.710999965667725
Features: marker(s): ampR
reporter group: luciferase (luc+)
terminator: SV40 large T-antigen polyadenylation
terminator: SV40 late polyadenylation
Restriction digests of the clone give the following sizes (kb): BamHI--4.7; HindIII--4.7.
The vector contains modified luciferase (luc+) gene from the firefly Photinus pyralis in order to increase the yield of recoverable luciferase activity after transfection and to eliminate potential cryptic regulatory elements.
The promoters driving luc+ transcription in these two vectors represent different 5' deletions of the tk promoter ending at -32 and -105, respectively.
The optimized luciferase reporter gene vectors (ATCC 87630
- 87633) provide valuable tools for the analysis of eukaryotic regulatory DNA elements.
The vectors pTATALUC+ (ATCC 87631)
and ptkLUC+ (ATCC 87632)
were designed for the characterization of isolated cis regulatory elements.
Altschmied J, Duschl J. Set of optimized luciferase reporter gene plasmids compatible with widely used CAT vectors. BioTechniques 23: 436-438, 1997. PubMed: 9298214