REP81X (ATCC® 87606)

Applications: expression vector  /  Depositors: SL Forsburg

Designations REP81X
Permits and Restrictions

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Depositors SL Forsburg
Biosafety Level 1
Vector Information
Size (kb): 8.8999996185302730
Vector: REP81X (phagemid)
Promoters: Promoter for expression nmt1** (much weaker nmt1)
Construction: pUC119
Construct size (kb): 8.899999618530273
Features: marker(s): LEU2
marker(s): ampR
promoter for expression: nmt1** (much weaker nmt1)
replicon: ars1
replicon: pMB1, f1
MCS: Xho...SmaI
expression vector
Restriction digests of the clone give the following sizes (kb): HindIII--5.6, 2.3, 1.0; EcoRI--4.4, 3.4, 1.1; XbaI--8.9.
REP3X (ATCC 87603) and REP4X (ATCC 87604) contain full strength nmt1 promoter whereas REP41X (ATCC 87605) and REP42X (ATCC 87607) with medium strength (nmt1*) and REP81X (ATCC 87606) and REP82X (ATCC 87608) with low strength (nmt1**)
due to mutation in the TATA box by Basi et al. (Gene 123:131-136, 1993).
REP81X (ATCC 87606) and REP82X (ATCC 87608) are different in that REP81X contains the LEU2 marker and REP82X, ura4+.
REP81X was constructed by deleting the ATG in the polylinker of the original REP81 plasmid. An XhoI linker was cloned into the blunted NdeI-BamHI cut REP81 plasmid, deleting SalI site.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Forsburg SL. Comparison of Schizosaccharomyces pombe expression systems. Nucleic Acids Res. 21: 2955-2956, 1993. PubMed: 8332516

Susan L Forsburg, personal communication

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