pRS416 (ATCC® 87521)

Applications: YC-type (centromeric) shuttle vector  /  Depositors: P Hieter

Permits and Restrictions

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Designations pRS416
Depositors P Hieter
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Distribution host: Escherichia coli DH5alpha
Vector Information
Size (kb): 4.898
Intact vector size: 4.898
Type of vector: phagemid
Cloning sites: KpnI ApaI XhoI SalI ClaI EcoRI PstI SmaI BamHI SpeI EagI
NotI SacII SacI NaeI
Polylinker sites: BssHII KpnI ApaI XhoI SalI ClaI HindIII EcoRV EcoRI PstI
SmaI BamHI SpeI XbaI EagI NotI BstXI SacII SacI BssHII
Construction: pBluescriptII KS+
Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli
Features (with orientation and position when available):
marker(s): URA3, ->, 417-1219
replicon: f1, <-, 1351-1807
insert detection: lacZ', <-, 1800-2137
promoter for in vitro transcription: T7, ->, 1977-1996
MCS: KpnI...SacI, ->, 2004-2111
promoter for in vitro transcription: T3, <-, 2123-2142
promoter: lac, <-, 2212-2240
replicon: pMB1, 2566-2566
marker(s): ampR, <-, 3324-4184
centromere: CEN6, 4326-4442
replicon: ARSH4, 4443-4806
Vector: pRS416 (phagemid)
Promoters: Promoter for in vitro transcription T7
Construction: pBluescriptII KS+
Construct size (kb): 4.898
Features: insert detection: lacZ'
marker(s): URA3
marker(s): ampR
promoter: lac
promoter for in vitro transcription: T3
promoter for in vitro transcription: T7
replicon: ARSH4
replicon: f1
replicon: pMB1
MCS: KpnI...SacI
centromere: CEN6
YC-type (centromeric) shuttle vector
Restriction digests of the clone give the following sizes (kb): PvuII--4.45, 0.45; BglI--3.2, 1.7; PstI--3.25, 1.65.
A YC-type shuttle vector with URA3 selection in Saccharomyces cerevisiae, a beta-galactosidase reporter system for visual detection of recombinants, promoters for transcription in vitro, and an extensive polylinker flanked by BssHII sites.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Sikorski RS, Hieter P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122: 19-27, 1989. PubMed: 2659436

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component of:ATCC 87534
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