pRS403 (ATCC® 87514)

Applications: YI-type (integrating) shuttle vector  /  Depositors: P Hieter

Permits and Restrictions

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Designations pRS403
Depositors P Hieter
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 4.456
Vector: pRS403 (phagemid)
Promoters: Promoter for in vitro transcription T7
Construction: pBluescriptII KS+
Construct size (kb): 4.456
Features: insert detection: lacZ'
marker(s): HIS3
marker(s): ampR
promoter: lac
promoter for in vitro transcription: T3
promoter for in vitro transcription: T7
replicon: f1
replicon: pMB1
MCS: KpnI...SacI
YI-type (integrating) shuttle vector
Restriction digests of the clone give the following sizes (kb): PvuII--4.0, 0.45; BglI--1.7, 1.7, 1.05; PstI--3.5, 0.95.
A YI-type shuttle vector with HIS3 selection in Saccharomyces cerevisiae, a beta-galactosidase reporter system for visual detection of recombinants, promoters for transcription in vitro, and an extensive polylinker flanked by BssHII sites.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Sikorski RS, Hieter P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122: 19-27, 1989. PubMed: 2659436

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