pRS421 (ATCC® 87475)

Applications: YE-type (episomal) shuttle vectorvector permitting visual detection of recombinants O-acetylhomoserine sulfhydrylase  /  Depositors: JD Boeke

Designations pRS421
Permits and Restrictions

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Depositors JD Boeke
Biosafety Level 1
Vector Information
Size (kb): 6.2329998016357420
Vector: pRS421 (phagemid)
Promoters: Promoter for in vitro transcription T7
Construction: pRS401, YEp24
Construct size (kb): 6.232999801635742
Features: insert detection: lacZ'
marker(s): MET15
marker(s): ampR
promoter: lac
promoter for in vitro transcription: T3
promoter for in vitro transcription: T7
replicon: 2 micron
replicon: f1
replicon: pMB1
MCS: KpnI...SacI
YE-type (episomal) shuttle vector
vector permitting visual detection of recombinants O-acetylhomoserine sulfhydrylase
Restriction digests of the clone give the following sizes (kb): BamHI--6.2; EcoRI--1.7, 4.5; XbaI--3.2, 2.0, 1.0.
met15 phenotype produces brown colonies when grown on Pb containing media.
MET15, MET17 and MET25 are synonymous.
This vector is useful for gene knockout experiments in hosts with a non-revertable met15 auxotrophic marker gene mutation.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Cost GJ, Boeke JD. A useful colony colour phenotype associated with the yeast selectable/counter-selectable marker MET15. Yeast 12: 939-941, 1996. PubMed: 8873447

Brachmann CB, et al. Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications. Yeast 14: 115-132, 1998. PubMed: 9483801

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Shipped frozen
Shipping Information

 Frozen glycerol stock of Escherichia coli containing the plasmid.