pRS411 (ATCC® 87474)

Applications: YC-type (centromeric) shuttle vector  vector permitting visual detection of recombinants O-acetylhomoserine sulfhydrylase  /  Depositors: JD Boeke

Permits and Restrictions

View Permits

Designations pRS411
Depositors JD Boeke
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Host
Distribution host: Escherichia coli HB101 (ATCC 33694)
Vector Information
Size (kb): 5.407
DESCRIPTION OF VECTOR:
Intact vector size: 5.407
Type of vector: phagemid
Cloning sites: SacI BstXI SacII EagI NotI SpeI BamHI SmaI PstI ClaI SalI
XhoI ApaI KpnI
Polylinker sites: SacI BstXI SacII EagI NotI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV ClaI SalI XhoI ApaI KpnI
Construction: pRS401, pRS84
Host range: Saccharomyces cerevisiae; Escherichia coli
Features (with orientation and position when available):
marker(s): MET15, ->, 421-1270
replicon: f1, <-, 1860-2316
promoter for in vitro transcription: T7, ->, 2486-2505
MCS: KpnI...SacI, ->, 2513-2615
promoter for in vitro transcription: T3, <-, 2632-2651
coding sequence: lacZ', <-, 2317-2676
promoter: lac, <-, 2721-2749
replicon: pMB1, 3075-3075
marker(s): ampR, <-, 3833-4693
centromere: CEN6, 4835-4951
replicon: ARS4, 4952-5353
Vector: pRS411 (phagemid)
Promoters: Promoter for in vitro transcription T7
Construction: pRS401, pRS84
Marker(s):ampR,MET15
Construct size (kb): 5.407
Features: marker(s): MET15
marker(s): ampR
promoter: lac
promoter for in vitro transcription: T3
promoter for in vitro transcription: T7
replicon: ARS4
replicon: f1
replicon: pMB1
MCS: KpnI...SacI
centromere: CEN6
coding sequence: lacZ'
Applications
YC-type (centromeric) shuttle vector 
vector permitting visual detection of recombinants O-acetylhomoserine sulfhydrylase
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--5.4; EcoRI--3.7, 1.7; XbaI--3.4, 2.0.
met15 phenotype produces brown colonies when grown on Pb containing media.
MET15, MET17 and MET25 are synonymous.
This vector is useful for gene knockout experiments in hosts with a non-revertable met15 auxotrophic marker gene mutation.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Cost GJ, Boeke JD. A useful colony colour phenotype associated with the yeast selectable/counter-selectable marker MET15. Yeast 12: 939-941, 1996. PubMed: 8873447

Brachmann CB, et al. Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications. Yeast 14: 115-132, 1998. PubMed: 9483801

Related Products
component of: ATCC 87476
component of: ATCC 87529
Shipped frozen
Shipping Information plasmid in E. coli
Product Sheet
Product Sheet
Product Sheet
Attachments Vector map for 87474