K Ippen-Ihler, S Maneewannakul
Size (kb): 3.5840001106262210
Vector: pKSM717 (phagemid)
Promoters: Promoter for in vitro transcription T7lac
Construction: pKSM710, pUC18
Construct size (kb): 3.584000110626221
Features: insert detection: lacZalpha
promoter for expression: lacUV5
promoter for in vitro transcription: T7lac
transcription terminator: T7
transcription terminator: fd
transcription terminator: rrnB
vector permitting RNA synthesis in vitro
vector permitting construction of fusion proteins
vector permitting production of single-stranded DNA
vector permitting visual detection of recombinants
Restriction digests of the clone give the following sizes (kb): BamHI--3.6; EcoRI--3.6; BglI--3.6.
Vector contains the following restriction sites (approximate kb from nt 1): BamHI--0.34; BglI--2.86; EcoRI--0.39; NcoI--0.33; PvuI--0.51, 3.11; PvuII--0.48.
Differs from pKSM718 (ATCC 87163)
only in the orientation of the ampicillin resistance gene.
Expression vector allowing generation of N-terminal deletions or modifications of foreign DNA cloned into the multiple cloning site.
Maneewannakul S, et al. The pKSM710 vector cassette provides tightly regulated lac and T7lac promoters and strategies for manipulating N-terminal protein sequences. Plasmid 31: 300-307, 1994. PubMed: 8058823