K Ippen-Ihler, S Maneewannakul
Size (kb): 3.5840001106262210
Vector: pKSM717 (phagemid)
Promoters: Promoter for in vitro transcription T7lac
Construction: pKSM710, pUC18
Construct size (kb): 3.584000110626221
Features: insert detection: lacZalpha
promoter for expression: lacUV5
promoter for in vitro transcription: T7lac
transcription terminator: T7
transcription terminator: fd
transcription terminator: rrnB
vector permitting RNA synthesis in vitro
vector permitting construction of fusion proteins
vector permitting production of single-stranded DNA
vector permitting visual detection of recombinants
Restriction digests of the clone give the following sizes (kb): BamHI--3.6; EcoRI--3.6; BglI--3.6.
Vector contains the following restriction sites (approximate kb from nt 1): BamHI--0.34; BglI--2.86; EcoRI--0.39; NcoI--0.33; PvuI--0.51, 3.11; PvuII--0.48.
Differs from pKSM718 (ATCC 87163)
only in the orientation of the ampicillin resistance gene.
Expression vector allowing generation of N-terminal deletions or modifications of foreign DNA cloned into the multiple cloning site.