Size (kb): 5.40
Vector: YCpIF15 (plasmid)
Promoters: Promoter for expression GAL1
Construction: YCpIF12, hemaglutinin epitope
Construct size (kb): 5.40
promoter for expression: GAL1
epitope tag: hemaglutinin (HA), influenza virus
YX-type (expression) shuttle vector
encodes an epitope tag for protein isolation or monitoring
Restriction digests of the clone give the following sizes (kb): XbaI--3.6, 1.8; BamHI--5.4; EcoRI--5.4.
Yeast expression vector allowing production of a protein tagged at the N-terminal end with a hemaglutinin epitope.
Vectors YCpIF15, YCpIF16 and YCpIF17 (ATCC 87090-87092) differ only in the reading frame of the multiple cloning site.
The encoded nine aa of the influenza virus hemaglutinin epitope are recognized by the monoclonal antibody 12CA5, allowing detection and affinity purification of the recombinant protein.
Foreman PK, Davis RW. Cloning vectors for the synthesis of epitope-tagged, truncated and chimeric proteins in Saccharomyces cerevisiae. Gene 144: 63-68, 1994. PubMed: 7517907