yWXD255 (ATCC® 79588)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: D Schlessinger

Permits and Restrictions

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Designations yWXD255
Species Homo sapiens, human
Depositors D Schlessinger
Vector
Construct size (kb): 350.0
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pYAC-RC
Intact vector size: 11.700
Type of vector: YAC
Vector end: ClaI
Vector end: ClaI
Cloning sites: NotI SacII SalI mLuI ClaI SnaBI SmaI
Polylinker sites: NotI SacII SalI mLuI ClaI SnaBI SmaI
Construction: pYAC3, polylinker
Host range: Saccharomyces cerevisiae; Escherichia coli
Features (with orientation and position when available):
centromere: CEN4
marker(s): ampR, HIS3, SUP4, TRP1, URA3
promoter: none
replicon: pMB1, ARS1
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: DXS809
Genomic copy number: unique
Gene name: DNA Segment, single copy
Contains complete coding sequence?: U
Chromosome: X; Localization: X q26
Genome: Homo sapiens
Gene symbol: F9
Genomic copy number: unique
Gene name: coagulation factor IX (plasma thromboplastic component,
Christmas disease, hemophilia B)
Contains complete coding sequence?: U
Chromosome: X; Localization: X q26.3-q27.1
Tissue: DNA from human/hamster cell line X3000.11
Type of DNA: genomic
Insert end: TaqI
Insert end: TaqI
Insert size (kb): 350.0
Cross references:
Insert lengths(kb): 350.0
Tissue: DNA from human/hamster cell line X3000.11
Gene product: coagulation factor IX (plasma thromboplastic component, Christmas disease, hemophilia B) [F9]
Alleles: A1, A2, D2, C1, D1, C2
Insert Size (kb) 350.0
Media ATCC® Medium 1798: aAHC medium for YAC clones
Related Products
component of:ATCC 77395
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: frozen
References

Abidi FE, et al. Yeast artificial chromosomes containing human Xq24-Xq28 DNA: library construction and representation of probe sequences. Genomics 7: 363-376, 1990. PubMed: 2365355

Little RD, et al. Yeast artificial chromosomes spanning 8 megabases and 10-15 centimorgans of human cytogenetic band Xq26. Proc. Natl. Acad. Sci. USA 89: 177-181, 1992. PubMed: 1729687

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