pT (ATCC® 77384)

Applications: expression vectorshuttle vectorvector permitting construction of fusion proteinsvector permitting visual detection of recombinants  /  Depositors: JJ McCormick

Designations pT
Permits and Restrictions

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Depositors JJ McCormick
Biosafety Level 1
Vector Information
Size (kb): 5.4229998588562000
Vector: pT (plasmid)
Promoters: Promoter lac
Construction: pUC19, pT24, SV40 early splicing region, poly(A) from pMAMneo
Construct size (kb): 5.4229998588562
Features: insert detection: lacZ'
marker(s): ampR
promoter: lac, metallothionein
replicon: pMB1
terminator: SV40
expression vector
shuttle vector
vector permitting construction of fusion proteins
vector permitting visual detection of recombinants
Restriction digests of the clone give the following sizes (kb): PvuI--3.6, 1.8; EcoRI--2.9, 2.5; HindIII--5.4; BglII--5.4; ClaI--5.4.
The plasmid contains the following restriction sites (bp from 0): NotI--0; EcoRI--9, 2894; SacI--1593; StyI--1627; ClaI--1814; XhoI--1836; BglII--1859; SphI--2730; NsiI--2732; PvuI--2774, 4564; HindIII--2945; DrdI--3406, 5277; BsaI--4264.
Expression vector containing two multiple cloning sites: the first interrupting the lacZ' gene, and the second being flanked by a murine metallothionein I promoter and splicing and polyadenylation signals.
The first site was designed for insertion of a marker selectable in mammalian cells, and the second for the sequence to be expressed. The first site permits detection of inserts by alpha complementation.
The order of the major features in this plasmid is: NotI - 5' metallothionein promoter 3' - ClaI/MCSII'/BglII - poly(A) - SphI - 3' lacZ' - EcoRI/MCSI/HindIII - 5' lacZ' - pMB1 ori - ampR.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Wang Q, et al. Mammalian expression vectors with modulatable promoters and two multiple cloning sites. Gene 119: 155-161, 1992. PubMed: 1327962

J J McCormick, personal communication

Shipped freeze-dried
Shipping Information  Freeze dried phagemid in E. coli