Size (kb): 4.2709999084472660
Vector: pRS304 (phagemid)
Promoters: Promoter T3
Construction: pRSS56 [pBluescript KS+, pBS(+)]
Construct size (kb): 4.270999908447266
Features: insert detection: lacZ'
marker(s): ampR, TRP1
promoter: lac, T3, T7
replicon: pMB1, f1
YI-type (integrating) shuttle vector
vector containing primer sites useful for sequencing
vector permitting RNA synthesis in vitro
vector permitting production of single-stranded DNA
vector permitting visual detection of recombinants
Restriction digests of the clone give the following sizes (kb): HindIII--3.3, 0.94; PvuII--2.8, 0.48; XbaI--2.8, 1.4; EcoRI--4.4.
One of a series of pBluescript-based integrating vectors (ATCC 77138-77141) differing in the yeast selectable marker gene.
YI-type integrating shuttle vector permitting visual detection of recombinants and production of ssDNA in E. coli. Contains promoters for in vitro RNA synthesis, priming sites useful for sequencing, and encodes the lacZ alpha (lacZ') peptide.
pRSS56, constructed by ligating a PvuI fragment (bp 498-2412) of pBluescript KS+ to a PvuI fragment (bp 2850-730) of pBS(+), contains the KS MCS from pBluescript KS+ and the unique NdeI and AatII sites between bla and f1 origin of pBS(+).
A genomic HincII/PstI fragment (1.002 kb) containing the TRP1 gene was inserted into the NdeI site of pRSS56. All ends were blunted and an internal EcoRI site was destroyed.
The order of the major features in this plasmid is: TRP1 - f1 ori (NaeI) - T7 promoter - lacZ'/MCS - T3 promoter - pMB1 ori - bla.
The following restriction sites in the multiple cloning site (MCS) are no longer unique: HindIII EcoRV XbaI BstXI.
Sikorski RS, Hieter P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122: 19-27, 1989. PubMed: 2659436