ATCC ATCC Logo 0
  • Quick Order
  • Careers
  • Support

pRSVlacZII

77129

Organism
Escherichia coli (Migula) Castellani and Chalmers
Classification
Enterobacteriaceae, Escherichia
Clone type
Vector
Applications
Molecular biology
Product format
Freeze-dried
Buy Now
Price: $620.00 EA
Discounts may be available for our fellow nonprofit organizations. Login to see your price.

Generally ships within 1-3 business days

Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Detailed product information

General

Specific applications
contains sequence beta-galactosidase beta-D-galactosidase
expression vector
in another host, produces protein beta-galactosidase beta-D-galactosidase
reporter construct
shuttle vector
vector permitting production of single-stranded DNA
vector permitting visual detection of activity in colonies

Characteristics

Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--4.4, 3.4, 1.2; HindIII--9.0; BglII--9.0, KpnI--4.5 (doublet); XbaI--9.0.
Transient expression experiments should be performed to examine the RSV promoter efficiency in different cell lines. The RSV promoter can be replaced by excising with a BglII/HindIII digest.
Shuttle reporter plasmid permitting visual detection of activity by histochemical staining.
Presence of beta-galactosidase activity is used to follow cell lineage in culture or in situ.
pRSVlacZII (ATCC 77129), pRSVPAP (ATCC 77130) and pRSVADH (ATCC 77131) provide distinct color-staining reactions (aqua blue, red, and blue-black respectively) to permit simultaneous analysis of multiple lineages.
The order of the major features in pRc/RSV is: RSV LTR - MCS - bovine growth hormone polyadenylation signal - M13 ori - SV40 early promoter - neoR - SV40 polyadenylation signal - pMB1 ori - bla.
Constructed by cloning the 3.9 kb HindIII/BamHI fragment from pM-MuLV-SV-lacZ into pRc/RSV.
Mycoplasma contamination
Not detected

Vector information

Construct size (kb)
8.899999618530273
Intact vector size
5.100
Vector name
pRc/RSV
Type of vector
phagemid
Construction
pUC19, RSV, bovine growth hormone; pRc/RSV, pM-MuLV-SV-lacZ
Host range
vertebrate cells
Vector end
HindIII; XbaI
Cloning sites
HindIII; SpeI; BstXI; NotI; XbaI
Enhancer
RSV LTR
Markers
neoR; G418R; ampR
Polylinker sites
HindIII; KpnI; SacI; BamHI; SpeI; XmaIII; BstXI; EcoRI; PstI; EcoRV; BstXI; NotI; XhoI; SphI; NsiI; XbaI
Promoters
RSV LTR
Replicon
pMB1; M13
Terminator
bGH polyadenylation

Insert information

Insert size (kb)
3.7000000000000002
Type of DNA
genomic
Insert information
Insert 5' end: KpnI
Insert 3' end: BamHI
Genome
Escherichia coli
Target gene
beta-galactosidase
Gene name
beta-galactosidase
Gene product
beta-galactosidase(beta-D-galactosidase) [lacZ]
Gene symbol
lacZ
Contains complete coding sequence
Unknown

Handling information

Host
Escherichia coli DH5alpha
Medium
Temperature
37°C
Handling notes
Restriction digests of the clone give the following sizes (kb): EcoRI--4.4, 3.4,
1.2; HindIII--9.0; BglII--9.0, KpnI--4.5 (doublet); XbaI--9.0.
- ATCC staff

Transient expression experiments should be performed to examine the RSV promoter
efficiency in different cell lines. The RSV promoter can be replaced by
excising with a BglII/HindIII digest.
- personal communication

Shuttle reporter plasmid permitting visual detection of activity by
histochemical staining.
- BioTechniques 11: 344-351, 1991

Presence of beta-galactosidase activity is used to follow cell lineage in
culture or in situ.
- BioTechniques 11: 344-351, 1991

pRSVlacZII (ATCC 77129), pRSVPAP (ATCC 77130) and pRSVADH (ATCC 77131) provide
distinct color-staining reactions (aqua blue, red, and blue-black respectively)
to permit simultaneous analysis of multiple lineages.
- BioTechniques 11: 344-351, 1991

The order of the major features in pRc/RSV is: RSV LTR - MCS - bovine growth
hormone polyadenylation signal - M13 ori - SV40 early promoter - neoR - SV40
polyadenylation signal - pMB1 ori - bla.
- BioTechniques 11: 344-351, 1991

Constructed by cloning the 3.9 kb HindIII/BamHI fragment from pM-MuLV-SV-lacZ
into pRc/RSV.
- BioTechniques 11: 344-351, 1991

History

Depositors
LA Culp

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

Frequently Asked Questions

References

Curated Citations

Lin WC, Culp LA. Selectable plasmid vectors with alternative and ultrasensitive histochemical marker genes. BioTechniques 11: 344-351, 1991. PubMed: 1931036

Sanes JR, et al. Use of a recombinant retrovirus to study post-implantation cell lineage in mouse embryos. EMBO J. 5: 3133-3142, 1986. PubMed: 3102226

Lloyd A Culp, personal communication

Need assistance with this product? Contact our Technical Support team.

Telephone

US and Puerto Rico
800-638-6597

Outside the US
+1-703-365-2700

Hours of Operation

Monday-Friday
9:00am - 5:00pm
US Eastern Time