pRX-1 (ATCC® 37677)

Applications: expression vectorvector permitting construction of fusion proteins  /  Depositors: TD Pollard

Designations pRX-1
Permits and Restrictions

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Depositors TD Pollard
Biosafety Level 1
Vector Information
Size (kb): 2.8499999046325680
Vector: pRX-1 (plasmid)
Promoters: Promoter trpE
Construction: pBR322, trpE, M13mp13 polylinker
Construct size (kb): 2.849999904632568
Features: marker(s): ampR
promoter: trpE
replicon: pMB1
MCS: EcoRI...ClaI
terminator: unknown terminator
expression vector
vector permitting construction of fusion proteins
Restriction digests of the clone give the following sizes (kb): EcoRI--2.85; ClaI--2.85.
When Escherichia coli CAG-456 (a protease deficient host) is used, this vector yields large amounts of the fusion protein (100 mg/liter or 45% of the soluble protein).
One of a series of plasmid expression vectors (ATCC 37677 to 37679) representing multiple reading frames for expression from the trpE promoter. The fusion proteins produced contain less than 25 amino acids of the trpE protein.
This vector contains the trpE operon and 51 bp of the trpE gene, followed by an 8-mer EcoRI linker and the M13mp13 multiple cloning site (MCS). There is a TGA stop codon (in frame from the GAA of the EcoRI site) 33 bp beyond the MCS ClaI site.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Rimm DL, Pollard TD. New plasmid vectors for high level synthesis of eukaryotic fusion proteins in Escherichia coli. Gene 75: 323-327, 1989. PubMed: 2653968

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