YRp7-17camp (ATCC® 37630)

Applications: YR-type (replicating) shuttle vectorshuttle vector  /  Depositors: H Feldmann

Designations YRp7-17camp
Permits and Restrictions

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Depositors H Feldmann
Biosafety Level 1
Vector Information
Size (kb): 6.8200001716613770
Vector: YRp7-17camp (plasmid)
Promoters: Promoter cml
Construction: YRp7, pBR325
Construct size (kb): 6.820000171661377
Features: marker(s): ampR, TRP1
promoter: cml
replicon: pMB1, ARS1
terminator: CAT
YR-type (replicating) shuttle vector
shuttle vector
Restriction digests of the clone give the following sizes (kb): SalI--7.0; EcoRI--4.6, 1.4, 0.82; PvuII--4.9, 2.05.
The depositor recommends using YRp7-17camp (ATCC 37630) as the reference for CAT expression and using the CAT assay methodology of Neumann, et al.
One of a series (ATCC 37624 through 37634) of Escherichia coli/Saccharomyces cerevisiae shuttle vectors for investigating control of expression in yeast.
A 5.47 kb ClaI/BamHI fragment of YRp7 was ligated to a 1.35 kb Sau96I fragment of pBR325. Contains the complete bacterial cml gene.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Mannhaupt G, et al. A series of shuttle vectors using chloramphenicol acetyltransferase as a reporter enzyme in yeast. Gene 67: 287-294, 1988. PubMed: 3049257

Shipped freeze-dried