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pCB4 (ATCC® 37272™)

Applications: vector for other uses  /  Depositors: HC Birnboim

Designations pCB4
Permits and Restrictions

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Depositors HC Birnboim
Biosafety Level 1
Vector Information
Size (kb): 0E-16
Vector: pCB4 (plasmid)
Construction: pBR322, Bacillus amyloliquefaciens
Marker(s):ampR
Construct size (kb): 0.0
Features: marker(s): ampR
replicon: pMB1
Applications
vector for other uses
Comments
Use with other pCB plasmids (ATCC 37270, 37274) as gel electrophoresis size markers for both covalently closed circular and linear DNA. 7322/14,644 bp.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Childs JD, Birnboim HC. Plasmids containing multiple copies of cloned DNA for use as DNA size markers for gel electrophoresis, constructed by bacteriophage T4 transduction. Plasmid 11: 82-91, 1984. PubMed: 6324261

Shipped freeze-dried