Tolypocladium inflatum Gams (ATCC® 34921)

Alternate State: Cordyceps subsessilis Petch, teleomorph  /  Strain Designations: NRRL 8044 [FERM-P 2796]  /  Product Format: freeze-dried

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Deposited As Trichoderma polysporum (Link : Persoon) Rifai
Strain Designations NRRL 8044 [FERM-P 2796]
Alternate State Cordyceps subsessilis Petch, teleomorph
Produces chitinase
Produces cyclosporin A S-7481/F-1
Produces cyclosporin B S-7481/F-2
Produces cyclosporin C
Produces cyclosporin D
Produces cyclosporin E
Produces cyclosporin G
Produces cyclosporin synthetase
Produces dihydrocyclosporin C
Produces dihydrocyclosporin G
Produces isocyclosporin G
Produces lipase
Transformation host
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Type Strain no
Preceptrol® no
Genome Sequenced Strain


Genome sequencing strain (Oregon State University, USA)
Medium ATCC® Medium 200: YM agar or YM broth
ATCC® Medium 336: Potato dextrose agar (PDA)
Growth Conditions
Temperature: 24°C to 26°C
Atmosphere: Typical aerobic
Sequenced Data
18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence


D1D2 region of the 28S ribosomal RNA gene


Name of Depositor NRRL
Cross References

Nucleotide (GenBank) : Z69598 CPA-element

Nucleotide (GenBank) : AJ295083 5.8S rRNA and ITS2

Nucleotide (GenBank) : Z28383 cyclosporin synthetase gene

Nucleotide (GenBank) : A36768 Sequence 1 from Patent EP0578616.

Nucleotide (GenBank) : A36772 Sequence 5 from Patent EP0578616.

Nucleotide (GenBank) : A40404 Sequence 31 from Patent WO9425606.

Nucleotide (GenBank) : A40406 Sequence 33 from Patent WO9425606.

Nucleotide (GenBank) : A40408 Sequence 35 from Patent WO9425606.

Nucleotide (GenBank) : A40409 Sequence 36 from Patent WO9425606.

Nucleotide (GenBank) : A40410 Sequence 37 from Patent WO9425606.

Nucleotide (GenBank) : S80937 lamda clone 6-14, repetitive element

Nucleotide (GenBank) : AJ251864 partial tnir1 gene for pathway-specific nitrogen regulator, exons 1-2

Nucleotide (GenBank) : AOHE00000000 Tolypocladium inflatum NRRL8044, whole genome shotgun sequencing project


Offenzeller M, et al. Biosynthesis of the unusual amino acid (4R)-4-[(E)-2-butenyl]-4-methyl-L-threonine of cyclosporin A. J. Biol. Chem. 268: 26127-26134, 1993. PubMed: 8253730

Ruegger A, Kuhn M. Dihydrocyclosporin C. US Patent 4,108,985 dated Aug 22 1978

Harri E, Ruegger A. Organic compounds. US Patent 4,117,118 dated Sep 26 1978

Traber RP, et al. Cyclosporin derivatives, their production and pharmaceutical compositions containing them. US Patent 4,288,431 dated Sep 8 1981

Weber G, et al. The peptide synthetase catalyzing cyclosporine production in Tolypocladium niveum is encoded by a giant 45.8-kilobase open reading frame. Curr. Genet. 26: 120-125, 1994. PubMed: 8001164

Weber G, Leitner E. Disruption of the cyclosporin synthetase gene of Tolypocladium niveum. Curr. Genet. 26: 461-467, 1994. PubMed: 7874740

von Arx JA. Tolypocladium, a synomym of Beauveria. Mycotaxon 25: 153-158, 1986.

Kempken F, et al. A unique repeated DNA sequence in the cyclosporin-producing strain of Tolypocladium inflatum (ATCC 34921). Exp. Mycol. 19: 305-313, 1995. PubMed: 8574905

Agathos SN, Lee J. Mathematical modeling of the production of cyclosporin A by Tolypocladium inflatum: effect of L-valine. Biotechnol. Prog. 9: 54-63, 1993. PubMed: 7763411

Kempken F, Kuck U. Evidence for circular transposition derivatives from the fungal hAT-transposon Restless. Curr. Genet. 34: 200-203, 1998. PubMed: 9745022

Lee TH, et al. Development of a cell-loaded biosupport separator for continuous immobilized-cell perfusion culture. Biotechnol. Prog. 15: 267-272, 1999. PubMed: 10194403

Ruegger A, et al. Cyclosporin A, a Peptide Metabolite from Trichoderma polysporum (Link ex Pers.) Rifai, with a remarkable immunosuppressive activity. Helv. Chim. Acta 59: 1075-1092, 1976. PubMed: 950308

Traber R, et al. New cyclopeptides from Trichoderma polysporum (Link ex Pers.) Rifai: cyclosporins B, D and E. Helv. Chim. Acta 60: 1568-1578, 1977. PubMed: 561044

Hoppert M, et al. Structure and localization of cyclosporin synthetase, the key enzyme of cyclosporin biosynthesis in Tolypocladiium inflatum. Arch. Microbiol. 176: 285-293, 2001. PubMed: 11685373

Lee J, Agathos SN. Effect of amino acids on the production of cyclosporin A by Tolypocladium inflatum. Biotechnol. Lett. 11: 77-82, 1989.

Aarnio TH, Agathos SN. Production of extracellular enzymes and cyclosporin by Tolypocladium inflatum and morphologically related fungi. Biotechnol. Lett. 11: 759-764, 1989.

Windhofer F, et al. Ds-like restless deletion derivatives occur in Tolypocladium inflatum and two foreign hosts, Neurospora crassa and Penicillium chrysogenum. Fungal Genet Biol 35: 171-182, 2002. PubMed: 11848679

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation