Cupriavidus necator Makkar and Casida (ATCC® 17697)

Strain Designations: 335 [CCUG 1776, CIP 104763, DSM 531, ICPB 3984, JCM 11282, LMG 1199, NCCB 82041, NCIB 11842, NRRL B-14690, NRRL B-4383, R-10-e]  /  Type Strain: yes  /  Biosafety Level: 1

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Deposited As Hydrogenomonas eutropha Wittenberg and Repaske
Strain Designations 335 [CCUG 1776, CIP 104763, DSM 531, ICPB 3984, JCM 11282, LMG 1199, NCCB 82041, NCIB 11842, NRRL B-14690, NRRL B-4383, R-10-e]
Application Produces poly-D-3-hydroxybutyric acid from carbon dioxide
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol® no
Type Strain yes
Comments Taxonomy
Dissimilation of aromatic compounds and regulation of beta-ketoadipate pathway
Medium ATCC® Medium 3: Nutrient agar or nutrient broth
Growth Conditions Temperature: 26°C
Atmosphere:   Aerobic
Name of Depositor RY Stanier
Chain of Custody
ATCC <-- RY Stanier <-- R Repaske R-10-e <-- C Bovell

Brenner DJ, et al. Validation list no. 57. Int. J. Syst. Bacteriol. 46: 625-626, 1996. PubMed: 8934914

Davis DH, et al. Proposal to reject the genus Hydrogenomonas: taxonomic implications. Int. J. Syst. Bacteriol. 19: 375-390, 1969.

Davis DH, et al. Taxonomic studies on some gram negative polarly flagellated "hydrogen bacteria" and related species. Arch. Mikrobiol. 70: 1-13, 1970. PubMed: 4987616

Goris J, et al. Classification of metal-resistant bacteria from industrial biotopes as Ralstonia campinensis sp. nov., Ralstonia metallidurans sp. nov. and Ralstonia basilensis Steinle et al.. Int. J. Syst. Evol. Microbiol. 51: 1773-1782, 2001. PubMed: 11594608

Hovanec TA, DeLong EF. Comparative analysis of nitrifying bacteria associated with freshwater and marine aquaria. Appl. Environ. Microbiol. 62: 2888-2896, 1996. PubMed: 8702281

Johnson BF, Stanier RY. Dissimilation of aromatic compounds by Alcaligenes eutrophus. J. Bacteriol. 107: 468-475, 1971. PubMed: 5113598

Johnson BF, Stanier RY. Regulation of the -ketoadipate pathway in Alcaligenes eutrophus. J. Bacteriol. 107: 476-485, 1971. PubMed: 5113599

Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.

Tanaka K, Ishizaki A. Production of poly-D-3-hydroxybutyric acid from carbon dioxide by a two-stage culture method employing Alcaligenes eutrophus ATCC 17697. J. Ferment. Bioeng. 77: 425-427, 1994.

Vaneechoutte M, et al. Wautersia gen. nov., a novel genus accommodating the phylogenetic lineage including Ralstonia eutropha and related species, and proposal of Ralstonia [Pseudomonas] syzygii (Roberts et al. 1990) comb. nov.. Int. J. Syst. Evol. Microbiol. 54: 317-327, 2004. PubMed: 15023939

Vandamme P, Coenye T. Taxonomy of the genus Cupriavidus: a tale of lost and found. Int. J. Syst. Evol. Microbiol. 54: 2285-2289, 2004. PubMed: 15545472

Yabuuchi E, et al. Transfer of two Burkholderia and an Alcaligenes species to Ralstonia gen. Nov.: Proposal of Ralstonia pickettii (Ralston, Palleroni and Doudoroff 1973) comb. Nov., Ralstonia solanacearum (Smith 1896) comb. Nov. and Ralstonia eutropha (Davis 1969) comb. Nov.. Microbiol. Immunol. 39: 897-904, 1995. PubMed: 8657018

Cross References

Nucleotide (GenBank) : M32021 A.eutrophus small subunit ribosomal RNA.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation