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Aspergillus niger van Tieghem (ATCC® 16888)

Strain Designations: WB 326 [CBS 554.65, IMI 50566, NRRL 326; 2766]  /  Product Format: freeze-dried

Permits and Restrictions

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Strain Designations WB 326 [CBS 554.65, IMI 50566, NRRL 326; 2766]
Produces aniline
Produces gluconic acid
Produces glucose oxidase
Produces glucosidase, beta; acid Glucosidase, beta, acid, glucocerebrosidase
Produces kynureninase-type enzymes
Produces pergolide sulfone
Produces pergolide sulfoxide
Produces pseudonigeron (1-3)-alpha-D-glucan
Degrades: pulp and paper-mill wastewater
Fermentation of tannin-gallic acid
Transformation of sesquiterpene lactone costunolide
Transformation of acetanilide to aniline
Transformation of pergolide to pergolide sulfoxide and pergolide sulfone
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Type Strain yes
Preceptrol® yes
Aspergillus reference strain for identification and morphological observation recommended by the International Commission on Penicillium and Aspergillus (ICPA)
Structure of galactomannan
Sequenced Data
18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence


D1D2 region of the 28S ribosomal RNA gene


Beta-tubulin (bTub)


Name of Depositor KB Raper, DI Fennell
Chain of Custody
ATCC <-- KB Raper, DI Fennell <-- NRRL 326 <-- C Thom 2766 <-- A Hollander
Isolation Not available.
Cross References

Nucleotide (GenBank) : KU729129 D1/D2 region of 28S rRNA gene

Nucleotide (GenBank) : FJ195350 ITS including 5.8S rRNA gene


Storm AM, Gasner LL. Treatment of pulp mill wastes. US Patent 3,737,374 dated Jun 5 1973

Klich MA, et al. Aspergillus reference cultures. New Orleans and Baarn: International Commission on Penicillium and Aspergillus; 1996.

Al-Musallam A Revision of the black Aspergillus species, 1980

Raper KB, Fennell DI. The genus Aspergillus. Baltimore: Williams & Wilkins; 1965.

Bardalaye PC, Nordin JH. Chemical structure of the galactomannan from the cell wall of Aspergillus niger. J. Biol. Chem. 252: 2584-2591, 1977. PubMed: 856795

Lasswell WL, et al. An undergraduate experiment in pharmacognosy: the microbial biotransformation of acetanilide by Aspergillus species. Am. J. Pharm. Educ. 47: 127-129, 1983.

Clark AM, Hufford CD. Microbial transformations of the sesquiterpene lactone costunolide. J. Chem. Soc. Perkin Trans. 1979: 3022-3028, 1979.

Smith RV, et al. Microbial transformations of pergolide to pergolide sulfoxide and pergolide sulfone. J. Pharm. Sci. 72: 733-736, 1983. PubMed: 6684155

Horisberger M, et al. Structure of A (1--->3) -alpha-D-glucan (pseudonigeron) of Aspergillus niger NRRL 326 cell wall. Carbohydr. Res. 23: 183-188, 1972. PubMed: 4340387

Witte K, Wartenberg A. Purification and properties of two beta-glucosidases isolated from Aspergillus niger. Acta Biotechnol. 9: 179-190, 1989.

Murado MA, et al. Enhancement of the bioproduction potential of an amylaceous effluent. Bioresour. Technol. 44: 155-163, 1993.

Shetty AS, Gaertner FH. Kynureninase-type enzymes of Penicillium roqueforti, Aspergillus niger, Rhizpus stolonifer, and Pseudomonas fluorescens: Further evidence for distinct kynureninase and hydroxykynureninase activities. J. Bacteriol. 122: 235-244, 1975. PubMed: 164432

FF MicroPlate. Biolog.

Lawrence DP, et al. Interkingdom gene transfer of a hybrid NPS/PKS from bacteria to filamentous Ascomycota. PLoS One 6: e28231, 2011. PubMed: 22140558

Andersen MR, et al. Comparative genomics of citric-acid-producing Aspergillus niger ATCC 1015 versus enzyme-producing CBS 513.88. Genome Res. 21: 885-897, 2011. PubMed: 21543515

Yamada O, et al. Molecular biological researches of Kuro-Koji molds, their classification and safety. J. Biosci. Bioeng. 112: 233-237, 2011. PubMed: 21641278

Peterson SW. Phylogenetic analysis of Aspergillus species using DNA sequences from four loci. Mycologia 100: 205-226, 2008. PubMed: 18595197

Geiser DM, et al. The current status of species recognition and identification in Aspergillus. Stud Mycol 59: 1-10, 2007. PubMed: 18490947

Serra R, et al. Aspergillus ibericus: a new species of section Nigri isolated from grapes. Mycologia 98: 295-306, 2006. PubMed: 16894975

Bau M, et al. DNA-based characterization of ochratoxin-A-producing and non-producing Aspergillus carbonarius strains from grapes. Res. Microbiol. 156: 375-381, 2005. PubMed: 15808942

Shimoda M, et al. Accelerated death kinetics of Aspergillus niger spores under high-pressure carbonation. Appl. Environ. Microbiol. 68: 4162-4167, 2002. PubMed: 12147527

Henry T, Iwen PC, Hinrichs SH. Identification of Aspergillus species using internal transcribed spacer regions 1 and 2. J. Clin. Microbiol. 38: 1510-1515, 2000. PubMed: 10747135

Perrone G, et al. Ochratoxin A production and amplified fragment length polymorphism analysis of Aspergillus carbonarius, Aspergillus tubingensis, and Aspergillus niger strains isolated from grapes in Italy. Appl. Environ. Microbiol. 72: 680-685, 2006. PubMed: 16391107

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation