Malassezia pachydermatis (Wiedman) Dodge, anamorph (ATCC® 14522)

Strain Designations: CBS 1879 [238 Shesis, CCRC 21676, IFO 10064, NRRL Y-17165]  /  Product Format: freeze-dried

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Deposited As Pityrosporum canis Gustafson
Strain Designations CBS 1879 [238 Shesis, CCRC 21676, IFO 10064, NRRL Y-17165]
Application
Biomedical Research and Development Material
Biosafety Level 2

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Type Strain yes
Preceptrol® no
Genome Sequenced Strain

Yes

Comments Genome sequencing strain (University of Utrecht, Netherlands)
Morphology After 15 days on Emmons’ modification of Sabouraud’s medium at 37°C, colonies are cream-colored, smooth, and soft. Yeast cells are ovoid, single, in pairs or clusters.
Sequenced Data
18S ribosomal RNA gene, partial sequence; internal   transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 26S ribosomal RNA gene, partial sequence.

GGTTTCTGTAGGTGAACCTGCAGAAGGATCATTAGTGAATTTGTAGGGCCTGCCATACGGATGCGCAAGCGTCTCTGGCGCCCAACCCACTATACATCCACAAACCCGTGTGCACTTGTGTTGCTTTGGCCTGTATGGGCGAGCGCACGCATTCAAACAAACTCGTATGGTTGTATGTACGTTGTAAACGTTGGACCGTCACTGGCCAACAAACTTTATACAACTTTCGACAACGGATCTCTTGGTTCTCCCATCGATGAAGAACGCAGCGAAACGCGATAGGTAATGTGAATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACCTTGCGCTCCATGGTATTCCGTGGAGCATGCCTGTTTGAGTGCCGCGAATTCTCCCACCCCAAACGGTTGCCGAAAGGTACTGTGCGGCGGAGGGGTTGGATGGGTGCTACTGCCTGTGGTGGGGAAACTACAACAGGCTCGCCCGAAATGCATTAGCGCCTCAGGACACATTCGCTACTGCTCTACAAAAGGAAGAGCAGCGAAGCGTATGGGGGGGAAAACCACCACCGGTTATTCCAAACCTCGCCTGACTGTTTTTGCGCGCATGGCATGATACGTCATTTGCTGTGTTGCGTAGGTTGGTGCGTGGGACTGTGTGAGTGCCTCTAGAGCTTTGAGAAGTGTGTTTCGTGTGCTACGAACTGAAAAAGTACATTCCTTTTTTTCAATTCTGGTCTCAAATCAGGTAGGATCACCCGCTGAACTTAAGCATATCAATAA


D1D2 region of the 26S ribosomal RNA gene.

ATATCAATAAGCGGAGGAGAAGAAACTAACAAGGATTCCCCTAGTAACGGCGAGCGAAGCGGGAAGAGCTCAAATTTGAAAGCTGGCACCTTCGGTGTCCGCGTTGTAATCTCGAGACGTGTTTTCCGTGTGGCGCTATGGACAAGTCCCTTGGAACAGGGCATCGTAGAGGGTGAAAATCCCGTACTTGCCATGGCTGTACCATGCTTTGTGATACACGCTCTAAGAGTCGAGTTGTTTGGGATTGCAGCTCAAAATGGGTGGTAGACTCCATCTAAAGCTAAATATCGGGGAGAGACCGATAGCGAACAAGTACCGTGAGGGAAAGATGAAAAGCACTTTGGAAAGAGAGTTAAAAGTACGTGAAATTGTCGAAAGGGAAGCGCTTGAAGTCAGCCATGCTGCTTGAGACTCAGCCTTGCTTTTGCTTGGTGTATTTCTCGGTAGCAAGCCAGCATTGGTTCGAGTCGTCGGAGAAGGGTATGAGAAATGTGGCATCCTCGGATGTGTTATAGACTTGTACTGGATACGACGATTTGGATCAAGGAACGCAGTGTGCCTCTGGCGGGTCTTCGGACACCTTCACACTTAGGATGCTGGCGTAATGGCTTTAAGCGGC

Morphology After 15 days on Emmons’ modification of Sabouraud’s medium at 37°C, colonies are cream-colored, smooth, and soft. Yeast cells are ovoid, single, in pairs or clusters.
Name of Depositor CBS
Chain of Custody
ATCC <-- CBS <-- BA Gustafson 238 Shesis
Isolation
Otitis externa in a dog, Sweden
Cross References

Nucleotide (GenBank) : AF063215 26S ribosomal RNA gene, partial sequence

Nucleotide (GenBank) : AJ249952 26S ribosomal RNA gene, partial sequence

Nucleotide (GenBank) : LGAV00000000 Malassezia pachydermatis strain CBS 1879, whole genome shotgun sequencing project

References

Lodder J. The yeasts: a taxonomic study. 2nd ed. Amsterdam: North-Holland; 1970.

Guillot J, Gueho E. The diversity of Malassezia yeasts confirmed by rRNA sequence and nuclear DNA comparisons. Antonie van Leeuwenhoek 67: 297-314, 1995. PubMed: 7778898

Fell JW, et al. Biodiversity and systematics of basidiomycetous yeasts as determined by large-subunit rDNA D1/D2 domain sequence analysis. Int. J. Syst. Evol. Microbiol. 50: 1351-1371, 2000. PubMed: 10843082

Triana S, et al. Draft genome sequence of the animal and human pathogen Malassezia pachydermatis strain CBS 1879. Genome Announc. 3(5): e01197-15, 2015. PubMed: 26472839

Castellá G, Coutinho SD, Cabañes FJ. Phylogenetic relationships of Malassezia species based on multilocus sequence analysis. Med. Mycol. 52: 99-105, 2014. PubMed: 23902157

Cabañes FJ, et al. Two New lipid-dependent Malassezia species from domestic animals. FEMS Yeast Res. 7(6): 1064-1076, 2007. PubMed: 17367513

Shibata N, et al. Isolation, characterization and molecular cloning of a lipolytic enzyme secreted from Malassezia pachydermatis. FEMS Microbiol. Lett. 256: 137-144, 2006. PubMed: 16487331

Sugita T, et al. DNA sequence diversity of intergenic spacer I region in the non-lipid-dependent species Malassezia pachydermatis isolated from animals. Med. Mycol. 43: 21-26, 2005. PubMed: 15712605

Cabanes FJ, Hernandez JJ, Castella G. Molecular analysis of Malassezia sympodialis-related strains from domestic animals. J Clin Microbiol 43: 277-283, 2005. PubMed: 15634983

Gupta AK, et al. Identification and typing of Malassezia species by amplified fragment length polymorphism and sequence analyses of the internal transcribed spacer and large-subunit regions of ribosomal DNA. J Clin Microbiol 42: 4253-4260, 2004. PubMed: 15365020

Yamada Y, et al. DNA base alignment and taxonomic study of genus Malassezia based upon partial sequences of mitochondrial large subunit ribosomal RNA gene. Microbiol. Immunol. 47: 475-478, 2003. PubMed: 12906109

Gaitanis G, et al. Identification of Malassezia species from patient skin scales by PCR-RFLP. Clin. Microbiol. Infect. 8: 162-173, 2002. PubMed: 12010171

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These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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