Epicoccum nigrum Link
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ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
Potential biocontrol agent: Fungal antagonist/mycoparasite
2. From a single test tube of sterile distilled water (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a sterile pipette and apply directly to the pellet. Stir to form a suspension.
3. Aseptically transfer the suspension back into the test tube of sterile distilled water.
4. Let the test tube sit at room temperature (25°C) undisturbed for at least 2 hours; overnight rehydration is recommended.
5. Mix the suspension well. Use several drops to inoculate recommended solid or liquid medium.
6. Incubate cultures at recommended temperature.
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Madrigal C, Melgarejo P. Morphological effects of Epicoccum nigrum and its antibiotic flavipin on Monilinia laxa. Can. J. Bot. 73: 425-431, 1995.
Madrigal C, et al. Biological control of peach twig blight (Monilinia laxa) with Epicoccum nigrum. Plant Pathol. 43: 554-561, 1994.
Madrigal C, et al. Relationship between flavipin production by Epicoccum nigrum and antagonism against Monilinia laxa. Mycol. Res. 95: 1375-1381, 1991.
Pascual S, et al. Production of the fungal biocontrol agent Epicoccum nigrum by solid substrate fermentation: effect of water activity on accumulation of compatible solutes. Mycopathologia 146: 83-89, 1999.