Ramularia beticola Fautrey et Lambotte
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1. To thaw a frozen ampoule, place in a 2530 °C water bath, until just thawed (approximately 5 minutes). Immerse the ampoule just sufficient to cover the frozen material. Do not agitate the ampoule.
2. Immediately after thawing, wipe down ampoule with 70% ethanol and aseptically transfer 10 microliter (or any amount desired up to all) of the content onto a plate or broth with medium recommended.
3. Incubate the inoculum/strain at the temperature and conditions recommended.
4. Inspect for growth of the inoculum/strain regularly. The sign of viability is noticeable typically after 5-7 days of incubation. However, the time necessary for significant growth will vary from strain to strain.
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Once you have the necessary permit, email the permit to [email protected] with a reference to both your account and sales order numbers. Once received, your permit will be reviewed, and this item will be released for shipment if all requirements are met. If you need assistance with your order, please contact our Customer Care team or your applicable distributor.
If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.
Hestbjerg H, et al. Development of Ramularia leaf spot on sugar beet as influenced by temperature and the age of the host plant. J. Phytopathol. 140: 293-300, 1994.