Alternaria alternata (Fries) Keissler
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2. From a single test tube of sterile distilled water (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette and use to rehydrate the pellet.
3. Aseptically transfer the rehydrated pellet back into the test tube with sterile distilled water. Mix well.
4. Let the test tube sit for at least 2 hours.
5. Mix the suspension well. Use several drops to inoculate a test tube of slant or a plate with recommended medium.
6. Incubate the test tube or plate at the temperature recommended.
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Portnoy J, et al. Selection of representative Alternaria strain groups on the basis of morphology, enzyme profile, and allergen content. J. Allergy Clin. Immunol. 91: 773-782, 1993. PubMed: 8454800