Cunninghamella elegans Lendner

9245 ^™

An ampoule containing viable cells (yeast cells, spores, or agar cubes with mycelia) suspended in cryoprotectant.

Product category: Fungi
Strain designation: 296 [CBS 167.53, DSM 1908, DSM 835, IMI 314507, NRRL 2310]
Type strain: No
Isolation source: Flax seeds, Linum usitatissimum
Geographical isolation: Canada; Manitoba; Winkler
Product format: Frozen
Storage conditions: -80°C or colder

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Documentation

Product sheet

BSL 1

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

General

Specific applications: hydrolyzes sclareol
produces 9,10-dimethyl-1,2 benzanthracene 7,12-dimethylbenz-[a]anthracene
produces acronine acronycine
produces carbomycin derivatives magnamycin derivatives
produces leucomycin derivatives
produces niddamycin derivatives
produces steroids
transforms amoxapine
transforms azatadine
transforms brompheniramine
transforms chlorpheniramine
transforms chlorpromazine
transforms methdilazine
transforms pheniramine
transforms solasodine
transformation of: amoxapine to 7-hydroxyamoxapine
transformation of: sesquiterpene lactone costunolide
transformation of: pergolide to pergolide sulfoxide
Preceptrol: No

Characteristics

Technical information: ATCC Technical Services does not have technical information on patent deposits that are not produced or characterized by ATCC. Additional information can be found in the corresponding patent available from the patent holder or with the U.S. and/or international patent office.

Handling information

Medium

ATCC Medium 200: YM agar or YM broth

ATCC Medium 323: Malt agar medium

ATCC Medium 336: Potato dextrose agar (PDA)

Temperature

24-26°C

Atmosphere

Aerobic

Handling procedure

Frozen ampoules packed in dry ice should either be thawed immediately or stored in liquid nitrogen. If liquid nitrogen storage facilities are not available, frozen ampoules may be stored at or below -70°C for approximately one week. Do not under any circumstance store frozen ampoules at refrigerator freezer temperatures (generally -20°C). Storage of frozen material at this temperature will result in the death of the culture.

To thaw a frozen ampoule, place in a 25°C to 30°C water bath, until just thawed (approximately 5 minutes). Immerse the ampoule just sufficient to cover the frozen material. Do not agitate the ampoule.
Immediately after thawing, wipe down ampoule with 70% ethanol and aseptically transfer at least 50 µL (or 2-3 agar cubes) of the content onto a plate or broth with medium recommended.
Incubate the inoculum/strain at the temperature and conditions recommended. Inspect for growth of the inoculum/strain regularly. The sign of viability is noticeable typically after 2-4 days of incubation. However, the time necessary for significant growth will vary from strain to strain.

Handling notes

No special notes

Additional, updated information on this product may be available on the ATCC web site at www.atcc.org.

Quality control specifications

Sequenced data

18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence

GGTTTCCGTAGGTGAACCTGCGGAAGGATCATTACTTATTCGGTCATTGGTTTTTATTCAAAAACCTTTGGCTTTAAATCATCCACAGTGTGGGAAATGTCTTCTAACGCTTGTGCCTGGTTCAGTCTAGTGCTGCCACTTGAGTTTACTCTTGGGTCAAGGGACCTTTGGGTAGTTTGTTCATTCGTGAGCAACCTCTTGTAACGGGGATAAGATTAATTTTATTATACTAAATTTTACTGAACTGATAGACCATAAATCTATGGTTGTTTTTTATTATAAACAAAAAAACAACTTTCAGCAATGGATCTCTCGGCTTTCGTATCGATGAAGAACGCAGCAAATCGCGATATGTAATGTGATCTGCCTATAGTGAATCATCAAATCTTTGAACGCATCTTGCACCTTATGGTATTCCATAAGGTACGTCTGTTTCAGTACCACTAGTAAATCTCCCCTCCACCTTGGTGGTTTAAAAGGAAGAGATAAATTATTACTGGTTCTGGTGATTCTTGATTTATTAAGAATTACTCTCGACCTAAATATAAGGCTCGACTTTTTTATTAGATCTCGCATCTGGTAAAACCTAGTCGGCTTTAATAGGATTTATTTCTTATTAGGTTTATAGCCATCATTTTACTTTTAAATCTTGGCCTGAAATCAGATGGGACTACCCGCTGAACTTAAGCATATCAATAA

D1D2 region of the 28S ribosomal RNA gene

CATATCAATAAGCGGAGGAAAAGAAAATAACAATGATTCCCCTAGTAACGGCGAGTGAAGAGGGAAAAGCTCAAAGTTGGAACCTGGTGGGCATAGCTCACCCGGATTGTAAACTAAAGTTTTTGAGTCGTTTAGTCAGCCAGGTAAATAAGTCCTCTGGAAAGGGGCGACATAGAGGGTGAAATCCCCGTCTTTGGCCTGAGTTTTGGTTAGGCGTTTGGCTTGGAAACGAAGAGTCAGGTTGTTTGGGAATGCAGCCTAAAATGGGAGGTAAATCTCTCCTAAAGCTAAATATTGACGAAAGACCGATAGCGAACAAGTACCGTGAGGGAAAGATGAAAAGCACTTTGAAAAGAGGGTCAAAAAGTACGTGAAATTGCTGAAAGGGAACCGTATGAAATCAGACCTACTGGTAGGTAATCAATCTTTCCCTTGGGAAGGATGCACTTGCCTGCTATGTATGCCAGCGACATTTTGGTTGGGAGGAAAAAAATAGAAGGAATGTAGCCTAGGCTTCGGTTTAGGTGTTATAGACTTTTATAAAATACTCTCGGCTGGAATGAGGAACGCAGCAAACCGTAAGGCGAAGATTCTAGTCGCTTGGGGGGAATAATTAGAGAATTTCTGCTTCGGGTGGTGCTTTGATTATTACTTTCAACTCGGTTGGAGTTCTTTTAATTTGCTTAGGTTGTTGGCTTAATGATTTTATATGA

History

Deposited as: Cunninghamella blakesleeana Lendner
Synonyms: Cunninghamella echinulata var. elegans (Lendner) Lunn et Shipton
Depositors: VM Cutter
Patent depository: This material was deposited with the ATCC Patent Depository to fulfill U.S. or international patent requirements. This material may not have been produced or characterized by ATCC. As an International Depository Authority (IDA) for patent deposits, ATCC is required to complete viability testing only at time of initial deposit of patent material. Patent deposits are made available on behalf of the Depositor when the pertinent U.S. or international patent is issued, but material may not be used to infringe the patent claims.
Type of isolate: Food & Beverage; Plant
Cross references: GenBank M36310 Cunninghamella elegans 5S ribosomal RNA.

Legal disclaimers

Intended use

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

Warranty

The product is provided 'AS IS' and the viability of ATCC^® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid. Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Disclosures

This material is cited in a US and/or international patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Depositor of the party to which the material was furnished.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Curated Citations

Bloom BM, Shull GM. Epoxidation of unsaturated steroids by microorganisms. J. Am. Chem. Soc. 87: 5767-5768, 1965.

Wong LK, et al. Metabolism of 7,12-dimethylbenz[a]anthracene by Cunninghamella elegans. Appl. Environ. Microbiol. 46: 1239-1242, 1983. PubMed: 6418073

Theriault RJ. Mycarosyl macrolide antibiotics. US Patent 3,784,447 dated Jan 8 1974

Wolf-Rainer A. Microbial hydroxylation of sclareol. Phytochemistry 36: 1421-1424, 1994.

Zhang D, et al. Biotransformation of chlorpromazine and methdilazine by Cunninghamella elegans. Appl. Environ. Microbiol. 62: 798-803, 1996. PubMed: 8975609

View All Curated Citations for this Product

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