Botrytis cinerea Persoon
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2. From a single test tube of sterile distilled water (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a sterile pipette and apply directly to the pellet. Stir to form a suspension.
3. Aseptically transfer the suspension back into the test tube of sterile distilled water.
4. Let the test tube sit at room temperature (25°C) undisturbed for at least 2 hours; overnight rehydration is recommended.
5. Mix the suspension well. Use several drops to inoculate recommended solid or liquid medium.
6. Incubate cultures at recommended temperature.
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Sahm H, et al. Purification and characterization of four extracellular 1,3-beta-glucanases of Botrytis cinerea. J. Gen. Microbiol. 139: 2833-2840, 1993.
Sahm H, et al. Degradation of extracellular þ-(1,3)(1,6)-D-glucan by Botrytis cinerea. Appl. Environ. Microbiol. 58: 3347-3354, 1992.
Stahmann KP, et al. Formation and degradation of the extracellular þ-(1,3)(1,6)-D-glucan by Botrytis cinerea. DECHEMA Biotechnol. Conf. 5: 117-120, 1992.