Mucor circinelloides f. lusitanicus Schipper
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2. From a single test tube of sterile distilled water (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a sterile pipette and apply directly to the pellet. Stir to form a suspension.
3. Aseptically transfer the suspension back into the test tube of sterile distilled water.
4. Let the test tube sit at room temperature (25°C
undisturbed for at least 2 hours; overnight rehydration is
5. Mix the suspension well. Use several drops to inoculate recommended solid or liquid medium.
6. Incubate cultures at recommended temperature.
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Roncero MI, et al. Characterization of a leuA gene and an ARS element from Mucor circinelloides. Gene 84: 335-343, 1989. PubMed: 2693214
Arnau J, et al. Integrative transformation of homologous recombination in the zygomycete Mucor circinelloides. Mol. Gen. Genet. 225: 193-198, 1991. PubMed: 2005861
Arnau J, Stroman P. Gene replacement and ectopic integration in the zygomycete Mucor circinelloides. Curr. Genet. 23: 542-546, 1993. PubMed: 8319313
Wolff AM, Arnau J. Cloning of glyceraldehyde-3-phosphate dehydrogenase-encoding genes in Mucor circinelloides (syn. racemosus) and use of the gpd1 promoter for recombinant protein production. Fungal Genet. Biol. 35: 21-29, 2002. PubMed: 11860262
Heeswijck RV, Roncero MI. High frequency transformation of Mucor with recombinant plasmid DNA. Carlsberg Res. Commun. 49: 691-702, 1984.