Epichloe festucae Leuchtmann et al., teleomorph
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1. To thaw a frozen ampoule, place in a 25-30 °C water bath, until just thawed (approximately 5 minutes). Immerse the ampoule just sufficient to cover the frozen material. Do not agitate the ampoule.
2. Immediately after thawing, wipe down ampoule with 70% ethanol and aseptically transfer at least 50 µl (or 2-3 agar cubes) of the content onto a plate or broth with medium recommended.
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Siegel MR, et al. Sexual compatibility and taxonomy of a new species of Epichloe symbiotic with fine fescue grasses. Mycologia 86: 802-812, 1994.