Phytophthora rubi (W.F. Wilcox & J.M. Duncan) Man in 't veld
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1. To thaw a frozen ampoule, place in a 2530 °C water bath, until just thawed (approximately 5 minutes). Immerse the ampoule just sufficient to cover the frozen material. Do not agitate the ampoule.
2. Immediately after thawing, wipe down ampoule with 70% ethanol and aseptically transfer at least 50 µl (or 2-3 agar cubes) of the content onto a plate or broth with medium recommended.
3. Incubate the inoculum/strain at the temperature and conditions recommended.
4. Inspect for growth of the inoculum/strain regularly. The sign of viability is noticeable typically after 5-6 days of incubation. However, the time necessary for significant growth will vary from strain to strain.
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Wilcox WF, et al. Identity of a Phytophthora species attacking raspberry in Europe and North America. Mycol. Res. 97: 817-831, 1993.
Olsson CH. Diagnosis of Phytophthora infections in raspberry and strawberry plants by ELISA tests. J. Phytopathol. 143: 307-310, 1995.