Candida albicans (Robin) Berkhout
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2. From a single test tube of sterile distilled water (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a sterile pipette and apply directly to the pellet. Stir to form a suspension.
3. Aseptically transfer the suspension back into the test tube of sterile distilled water.
4. Let the test tube sit at room temperature (25º C) undisturbed for at least 2 hours; overnight rehydration is recommended.
5. Mix the suspension well. Use several drops to inoculate appropriate solid or liquid media (see atcc.org for a recommended medium and temperature).
6. Incubate cultures at appropriate temperature. Remaining suspension may be stored in refrigerator for possible reuse, although no survival time can be specified.
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Turner JR, et al. A32390A, a new biologically active metabolite. III. In vitro and in vivo antifungal activity. J. Antibiot. 31: 33-37, 1978. PubMed: 342474
Turner JR, et al. Colonization of the intestinal tract of conventional mice with Candida albicans and treatment with antifungal agents. Antimicrob. Agents Chemother. 9: 787-792, 1976. PubMed: 782356
Debono M, et al. Synthesis and evaluation of LY121019, a member of a series of semisynthetic analogues of the antifungal lipopeptide echinocandin B. Ann. N.Y. Acad. Sci. 544: 152-167, 1988. PubMed: 3063167
Gordee RS, et al. In vitro and in vivo anti-Candida activity and toxicology of LY121019. J. Antibiot. 37: 1054-1065, 1984. PubMed: 6389460