Meyerozyma guilliermondii (Wickerham) Kurtzman et M. Suzuki
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Potential biocontrol agent: Control post-harvest diseases of fruits and vegetables
2. From a single test tube of sterile distilled water (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a sterile pipette and apply directly to the pellet. Stir to form a suspension.
3. Aseptically transfer the suspension back into the test tube of sterile distilled water.
4. Let the test tube sit at room temperature (25°C) undisturbed for at least 2 hours; overnight rehydration is recommended.
5. Mix the suspension well. Use several drops to inoculate recommended solid or liquid medium.
6. Incubate at recommended temperature.
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Wilson CL, Chalutz E. Pichia guilliermondii (anamorph Candida guillermondii) useful for the biological control of postharvest rots in fruits. US Patent 5,041,384 dated Aug 20 1991