Aspergillus parasiticus Speare
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ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
2. From a single test tube of sterile distilled water (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette and use to rehydrate the pellet.
3. Aseptically transfer the rehydrated pellet back into the test tube with sterile distilled water. Mix well.
4. Let the test tube sit for at least 2 hours.
5. Mix the suspension well. Use several drops to inoculate a test tube of slant or a plate with recommended medium.
6. Incubate the test tube or plate at the temperature recommended.
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If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.
McCormick SP, et al. Averufanin is an aflatoxin B1 precursor between averantin and averufin in the biosynthetic pathway. Appl. Environ. Microbiol. 53: 14-16, 1987. PubMed: 3103529
Dorner JW, et al. The relationship of Aspergillus flavus and Aspergillus parasiticus with reference to production of aflatoxins and cyclopiazonic acid. Mycopathologia 87: 13-15, 1984. PubMed: 6436707
Chang PK, et al. Cloning of the Aspergillus parasiticus apa-2 gene associated with the regulation of aflatoxin biosynthesis. Appl. Environ. Microbiol. 59: 3273-3279, 1993. PubMed: 8250554