Rhizoctonia solani Kühn
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1. To thaw a frozen ampoule, place in a 2530 °C water bath, until just thawed (approximately 5 minutes). Immerse the ampoule just sufficient to cover the frozen material. Do not agitate the ampoule.
2. Immediately after thawing, wipe down ampoule with 70% ethanol and aseptically transfer 10 microliter (or any amount desired up to all) of the content onto a plate or broth with medium recommended.
3. Incubate the inoculum/strain at the temperature and conditions recommended.
4. Inspect for growth of the inoculum/strain regularly. The sign of viability is noticeable typically after 1-2 days of incubation. However, the time necessary for significant growth will vary from strain to strain.
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Carling DE, Leiner RH. Isolation and characterization of Rhizoctonia solani and Binucleate R. solanii-like fungi from aerial stems and subterranean organs of potato plants. Phytopathology 76: 725-729, 1986.
Carling DE, et al. Characterization of a new anastomosis group (AG-9) of Rhizoctonia solani. Phytopathology 77: 1609-1612, 1987.