Ogataea methanolica (Makiguchi) Kurtzman et Robnett
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2. From a single test tube of sterile distilled water (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette and use to rehydrate the pellet.
3. Aseptically transfer the rehydrated pellet back into the test tube with sterile distilled water. Mix well.
4. Let the test tube sit for at least 2 hours.
5. Mix the suspension well. Use several drops to inoculate a test tube of slant or a plate with recommended medium.
6. Incubate the test tube or plate at the temperature recommended.
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Urakami T, Michimi R. Process for producing yeast cells. US Patent 4,033,821 dated Jul 5 1977
Urakami T, et al. Isolation, identification and cultivation of methanol-utilizing yeasts. J. Ferment. Technol. 61: 221-231, 1983.